Crystallization (Comment) | Organism |
---|---|
mutant enzyme R188I alone or in complex with several inhibitors, sitting drop vapor diffusion method, using 9-11% (w/v) of PEG 20000, 100 mM MES, pH 6.0, and 5 mM dithiothreitol | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
R188I | the enzymatic efficiency of the R188I mutant is increased by a factor of more than 1 million | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by N-acetyl-Ser-Ala-Val-Leu-NHCH(CH2CH2CON(CH3)2)-CH2CH-CHCOOEt and N-acetyl-Ser-Ala-Val-Leu-NHCH(CH2CH2CON(CH3)2)-(CH2)2-CH-CHCOOEt | Severe acute respiratory syndrome-related coronavirus | |
N-(2-[2-[(2S)-1-cyclohexyl-3-oxopropan-2-yl]hydrazinyl]-4-methylpentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | - |
Severe acute respiratory syndrome-related coronavirus | |
N-(2-[2-[(2S)-3,3-dimethyl-1-oxobutan-2-yl]hydrazinyl]-4-methylpentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | - |
Severe acute respiratory syndrome-related coronavirus | |
N-(4-methyl-2-[2-[(2S)-1-oxo-3-(thiophen-2-yl)propan-2-yl]hydrazinyl]pentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | - |
Severe acute respiratory syndrome-related coronavirus | |
N-(4-methyl-2-[2-[(2S)-1-oxo-3-phenylpropan-2-yl]hydrazinyl]pentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ala-Val-Cha-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Asn-Val-Cha-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Cha-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Leu-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Leu-NHCH(CH2CH2CON(CH3)2)-CH=CHCOOEt | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Leu-NHCH-(CH2CH2CON(CH3)2)-CHO | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Leu-NHCH[CH2CH2CON(CH3)2]CH2CH2CH=CHCOOC2H5 | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Ala-Val-Phe-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Ser-Val-Cha-His | - |
Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-Thr-Val-Cha-His | - |
Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | P0C6U8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-NH2 + H2O | - |
Severe acute respiratory syndrome-related coronavirus | H-Thr-Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
chymotrypsin-like protease | - |
Severe acute respiratory syndrome-related coronavirus |
SARS 3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000065 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Ala-Val-Cha-His | |
0.00027 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ala-Val-Cha-His | |
0.00034 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Val-Cha-His | |
0.00039 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Ala-Val-Phe-His | |
0.00098 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Thr-Val-Cha-His | |
0.0057 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Ala-Val-Leu-His | |
0.01 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Asn-Val-Cha-His | |
0.037 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Ala-Val-Leu-NHCH-(CH2CH2CON(CH3)2)-CHO | |
0.048 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-(4-methyl-2-[2-[(2S)-1-oxo-3-(thiophen-2-yl)propan-2-yl]hydrazinyl]pentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | |
0.062 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-(2-[2-[(2S)-1-cyclohexyl-3-oxopropan-2-yl]hydrazinyl]-4-methylpentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | |
0.33 | - |
in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-acetyl-Ser-Ala-Val-Leu-NHCH(CH2CH2CON(CH3)2)-CH=CHCOOEt | |
2 | - |
IC50 about 2 mM, in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-(4-methyl-2-[2-[(2S)-1-oxo-3-phenylpropan-2-yl]hydrazinyl]pentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide | |
3 | - |
IC50 about 3 mM, in 10 mM Tris-HCl buffer, pH 7.5, containing 7 mM dithiothreitol, at 37°C | Severe acute respiratory syndrome-related coronavirus | N-(2-[2-[(2S)-3,3-dimethyl-1-oxobutan-2-yl]hydrazinyl]-4-methylpentanoyl)valyl-N-(1-hydroxy-3,4-dioxopentan-2-yl)alaninamide |